Dimethyl disulfide produced in sterile fish muscle by Pseudomonas putrefaciens Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/z029p785m

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  • Sterile fish muscle homogenate was prepared from the individually line caught black rockfish (Sebastes melanops) and inoculated with the pure culture of Pseudomonas putrefaciens strain 17. The inoculated homogenate was incubated at 5°C and the growth and production of volatile sulfur compounds determined by a combined gas-liquid chromatography and mass spectrometry. A column containing 90-100 mesh diatomaceous earth coated with 10% Carbowax 20M was developed and tested for the quantitative determination of dimethyl sulfide (DMS), dimethyl disulfide (DMDS) and dimethyl trisulfide (DMTS). The DMDS levels in fish homogenate closely paralleled that of P. putrefaciens growth. The maximum DMDS level of 2.00 μg per 100 g fish homogenate was obtained after 10 days at 5°C in samples treated with 1.5% NaCl. NaCl was not required by P. putrefaciens but both the growth and DMDS production were stimulated by 1-2% of NaCl. The DMDS production and the growth of P. putrefaciens were reduced when the homogenate was treated with sodium benzoate (SB) or ethylenediaminetetraacetic acid (EDTA). The maximum levels of DMDS in 0.05% SB treated fish were 0.75 μg per 100 g and 0.85 μg for 0.05% EDTA treatment, respectively. The SB inhibited the growth rate as well as the maximum growth of P. putrefaciens, while EDTA had no effect on the maximum growth but extended the lag period and reduced the rate of growth. Potassium sorbate (PS) had little effect on DMDS production or the growth of P. putrefaciens. The maximum level of DMDS in fish homogenate, treated with 0.1% PS, was 1.80 μg per 100 g and the growth curve was similar to that of the control.
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