Graduate Thesis Or Dissertation
 

An histochemical study of the changing patterns of glycogen distribution in the estrus cycle and post partum (non lactation) uterus of the golden hamster (Mesocricetus auratus Waterhouse)

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  • This histochemical study deals with the changing patterns of glycogen in the uterus of the immature virgin, cycling virgin, and post partum (non-lactating) golden hamster (Mesocricetus auratus Waterhouse). The periodic acid-Schiff technique was used for the histochemical localization of glycogen in the uterus of the immature, cycling and non-lactating post partum (days 1 through 10, 12, and 14) hamster. Diastase controls were employed to determine the specific sites of glycogen throughout this organ. During the sexual cycle, the total uterine glycogen content is low in both metestrus and diestrus uteri, slightly higher in the proestrus uterus, and maximal in the estrus uterus. The glycogen content in every phase of the cycle exceeds that found in the immature hamster uterus. In proestrus, glycogen is present in the outer myometrium and blood vessel tunic in moderate amounts, and in minimal amounts in the inner myometrium, endometrial glands, and uterine lumen. The uterine epithelium stains lightly for diffuse glycogen at this time. During estrus, glycogen increases to large amounts in both the endometrial glands and uterine lumen, but to small amounts at most in both the inner myometrium and uterine epithelium. Glycogen remains in moderate amounts in the outer myometrium and blood vessel tunic. Decreases in glycogen content are seen in all areas of the uterus during metestrus: the outer myometrium contains small amounts of glycogen; the endometrial glands, vascular tunic and uterine lumen all demonstrate minimum amounts of glycogen; and both the inner myometrium and uterine epithelium stain lightly for diffuse glycogen. During diestrus, glycogen content increases slightly in both the inner myometrium and uterine lumen, but remains unchanged in the outer myometrium, blood vessel tunic, and uterine epithelium. The endometrial glands contain no glycogen at this time. Throughout the estrus cycle, glycogen is either absent, or present in varying amounts of the diffuse form in both the endometrial stroma and basement membrane of the uterine epithelium. No glycogen is present in the perimetrium during the estrus cycle. During the first six days after parturition, there is a rise in the amounts of myometrial glycogen above those found in the myometrium of the uteri of both cycling and immature hamsters. Throughout the rest of the post partum period however, myometrial glycogen deposits return to estrus cycle values. The vascular tunic contains markedly more glycogen during the first seven days post partum than in the last half of the period studied, while glycogen content in the uterine lumen fluctuates greatly during the post partum period studied. Relatively insignificant amounts of glycogen occur in the endometrial stroma, endometrial glands, and the uterine epithelium and associated basement membrane, throughout this period. Glycogen is absent from the perimetrium during this post partum period. In comparison with the amount of uterine glycogen at term as given by York and Hillemann (1968), the post parturient content is relatively lower; however, during the first seven days after parturition, the glycogen levels are still above those found in the uteri of all cycling hamsters, except those in estrus. Throughout the post partum period studied, total uterine glycogen is slightly higher in amount in non-lactating (post partum) animals, than in the lactating post partum animals studied by Wicklund (1968). The suggested relationship of glycogen and uterine energy requirements is discussed.
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