Nitrogen fixation associated with grasses and other species in Oregon Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/z890rz019

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  • Nitrogen fixation in both natural and domestic grasslands of Oregon was studied using the acetylene reduction assay. A substantial number of these tests made gave positive acetylene reduction activity. Rates in the range of 60g N₂ fixed/ha/day were observed for some Agrostis species. Assuming that this rate proceeds for 100 days during a year, 6 kg of nitrogen/ha/year would be made available for plant growth. It is concluded that this quantity of nitrogen would be of considerable importance in the maintenance of this species under natural conditions. Anaerobic or facultatively anaerobic nitrogen fixing microorganisms were isolated from the surfaces of roots. Pure cultures of Bacillus macerans, Bacillus polymyxa, and Enterobacter cloacae were isolated from wheat roots. Two other nitrogen fixing microorganisms were also isolated and characterized, but these have not been assigned a specific taxonomic classification.
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