Graduate Thesis Or Dissertation
 

Reproduction in chinchilla : superovulation

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  • The object of this study was to investigate the possibilities of inducing superovulation in the chinchilla (Chinchilla lanigera) by establishing the optimum dosage of pregnant mares' serum (PMS) to produce a maximum ovulatory response, and to compare chinchilla with other animals in this respect. Non-pregnant female chinchillas were given a single subcutaneous injection of 1.0, 1.2, 1.5, 1.6, 1.8, 2.0, 3.3, 4.0, 8.0 or 16.0 C-N units of PMS. The ovaries of these females were examined to determine the effect of PMS on ovarian weight, on the number of small follicles, large follicles, accessory corpora lutea, and corpora lutea of ovulation. The data were compared with those of Hillemann, Tibbitts, and Gaynor (1959) for untreated chinchillas. Following PMS treatment an increase was observed in ovarian weight, in the number of small follicles, accessory corpora lutea, and corpora lutea of ovulation, but not in the number of large follicles. The higher PMS doses induced also an increase in the number of cystic follicles. In terms of over-all ovarian response, and especially in terms of the ovulatory response, it appears that the optimum PMS dose for chinchillas is 1.6 C-N units. From an analysis of the percent of animals exhibiting an increase in ovulatory response, and of the extent of ovulation in individual animals, it appears that the best response is achieved when PMS is given in the follicular phase of the estrous cycle. Evidently anestrous females cannot be stimulated to ovulate with PMS. A number of other questions requiring further clarification are discussed.
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file details DorschJohn1965.pdf 2017-08-04 Pubblico Scaricare