Graduate Thesis Or Dissertation

 

Improvement of cryopreservation regrowth of in vitro Ribes by pretreatment Public Deposited

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  • Improved recovery of cryopreserved meristems and calli from in vitro currant (Ribes aureum Pursh and R. ciliatum Humb. & Bonpl.) plants was obtained by two-hour pretreatment with sucrose, proline, abscisic acid-responsive protein (RABP), and bovine serum albumin (BSA) solutions. Meristems and calli cold-acclimatized for one week were immersed in pretreatment solutions for two hours prior to cryopreservation by vitrification. Pretreatment with 5% proline, 1% crude RABP, 0.2% dialyzed RABP, and 1% BSA significantly increased the regrowth, compared to pretreatment with 0.4 M sucrose liquid NCGR-RIB medium, and non-pretreated controls. There were no significant differences among proline, crude and dialyzed RABP, and BSA pretreatments. Meristems in pretreatment groups resumed growth three days after thawing, and reached the maximum regrowth at one week, compared to two weeks for non-pretreated controls. Pretreatment by hourly immersion provides a simple and effective approach for improving the recovery of cryopreserved meristems and calli. Improved regrowth of cryopreserved meristems was also obtained by cold acclimatization (CA) of plantlets before dissection of meristems. Meristems acclimatized for 3 or 4 wk had more regrowth than those acclimatized for 1 or 2 wk. No significant differences were found between 1 and 2 wk, and 3 and 4 wk CA pretreatments.
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