Honors College Thesis
 

Identification of the Polycomb Repressive Complex 2 in Fungi

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  • The process of expressing a gene involves not just binding of proteins and enzymes to DNA to produce first RNA and eventually proteins, but rather association of these transcription factors with chromatin and structural proteins called histones. When histone H3 is trimethylated at lysine 27 to produce H3K27me3, gene silencing is found. It is known that a protein complex called Polycomb Repressive Complex 2 (PRC2) allows said trimethylation to take place, with kmt6 being a key subunit to carry out the process. Mutants who lack this gene lack the H3K27me3 marker and suffer from slow growth and infertility. In this experiment, other subunits of PRC2 are studied (caf1-3, suz12, and eed) in the fungus Fusarium graminearum. The last two subunits mentioned are found to be crucial to the fungus just as kmt6 was, with the result of mutants that lack the genes for those subunits exhibit similar observations as that of the kmt6 mutant. Further studies can confirm how these subunits are interacting with each other and their specific roles in histone methylation and in turn, gene silencing.
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  • Howard Hughes Medical Institute; Undergraduate Research, Innovation, Scholarship & Creativity (URISC); CURE
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file details Phuong Pham HHMI 2012 Symposium Presentation .pdf 07/27/2017 Public
file details Phuong Pham HHMI 2012 Symposium Presentation .pptx 07/27/2017 Public