Honors College Thesis
 

Analysis of Mercury Content in Dried Urine of Individuals with Amalgam Fillings Through theUtilization of Direct Mercury Analysis

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  • Mercury exposure is widely common throughout the world, ranging from sources such as dental amalgams, fish and seafood consumption, industrial mining, and medicine. A straightforward, efficient, and affordable protocol to measure urine mercury content would greatly contribute to assessing one’s mercury exposure and in categorizing populations that are more vulnerable to such exposure. The common current methods of mercury testing require a large amount of sample digestion time, sample quantity, and are extremely costly. Therefore, this project developed an assay that utilizes dried urine strips for direct mercury analysis, evading the limitations of time, cost, and sample quantity. To test the effectiveness of the direct mercury analysis method, dried urine samples were collected from individual’s with various numbers of amalgam fillings and tested for mercury concentration. Results demonstrated that there is a direct correlation between number of amalgams and urine mercury content and that the direct mercury analysis assay is ideal for measuring urine mercury content and for further clinical applications and research.
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