Honors College Thesis

 

Screening for Viral Disease in the OSU Tomato Breeding Program Public Deposited

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https://ir.library.oregonstate.edu/concern/honors_college_theses/3b591f614

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  • Disease control is a crucial aspect of plant breeding for researchers as well as commercial producers. After samples from OSU's tomato breeding program tested positive for Columnea latent viroid in a screen done by a private company, secondary testing was done by the USDA laboratory in Corvallis which suggested that the samples were infected with tobamovirus instead. This project was set up to screen symptomatic plants for both Columnea latent viroid as well as tobamovirus using reverse transcription polymerase chain reaction (RT-PCR). Out of the 42 samples tested, one sample from the P321-16-1B-1 line tested positive for tobamovirus, and one sample from the S261 line tested positive for Columnea latent viroid. However, sequencing of the S261 positive indicated that the primer used in that test had amplified tomato DNA and was therefore a false positive.
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