Screening for Viral Disease in the OSU Tomato Breeding Program

Honors College Thesis

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Citeable URL: https://ir.library.oregonstate.edu/concern/honors_college_theses/3b591f614

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Attribute Name	Values
Creator	Kershner, Alexandria C.
Abstract	Disease control is a crucial aspect of plant breeding for researchers as well as commercial producers. After samples from OSU's tomato breeding program tested positive for Columnea latent viroid in a screen done by a private company, secondary testing was done by the USDA laboratory in Corvallis which suggested that the samples were infected with tobamovirus instead. This project was set up to screen symptomatic plants for both Columnea latent viroid as well as tobamovirus using reverse transcription polymerase chain reaction (RT-PCR). Out of the 42 samples tested, one sample from the P321-16-1B-1 line tested positive for tobamovirus, and one sample from the S261 line tested positive for Columnea latent viroid. However, sequencing of the S261 positive indicated that the primer used in that test had amplified tomato DNA and was therefore a false positive.
License	All rights reserved
Resource Type	Honors College Thesis
Date Issued	2018-05-30
Degree Level	Bachelor's
Degree Name	Honors Bachelor of Science (H.B.S.)
Degree Field	Biology
Degree Grantor	Oregon State University
Commencement Year	2018
Advisor	Myers, Jim
Committee Member	Keller, Karen Burgett, Michael
Non-Academic Affiliation	Oregon State University. Honors College
Subject	Tomatoes -- Diseases and pests
权利声明	In Copyright
Publisher	Oregon State University
Peer Reviewed	No
Language	English [eng]

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