Honors College Thesis
 

Controlled Orientation of Engineered Carbonic Anhydrase on a Surface : A Model for Protein Immobilization for Biosensors and Biomaterials

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https://ir.library.oregonstate.edu/concern/honors_college_theses/41687k03g

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  • Current protein immobilization techniques lack defined control over protein orientation. Protein orientation is important for making biosensors and biomaterials that are sensitive and efficient and can be crucial for designing some devices such as immunosensors or direct electron transferring biocells. A new method of protein immobilization is proposed that takes advantage of amber stop codon suppression to incorporate a tetrazine containing non-canonical amino acid that can undergo an inverse-electron demand Diels-Alder cycloaddition with strained trans-cyclooctene-labelled surfaces. This allows for site specific protein immobilization that is fast, biorthogonal, and effective in mild aqueous conditions. This new method is compared to standard glutaraldehyde immobilization to lysine residues and shown to be faster and retain more activity when immobilized.
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  • 2017-07-27 to 2021-06-10

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file details BrownWesJ2016.pdf 2017-07-27 Public Download