Honors College Thesis
 

Analysis and Modeling of Kinetic Parameters for Urea Hydrolysis by Bacteria for Use in Calcium Carbonate Precipitation in Aquifers

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  • The use of ureolytic bacteria for the remediation of contaminated groundwater aquifers by inducing calcium carbonate precipitation is being studied in order to establish a better understanding of the modeling and prediction of how the bacteria will act in situ. This research has pursued the use of various ureolytic bacteria in order to establish a comparison for selection of model bacteria. The naturally ureolytic bacterium Sporosarcina pasteurii performed at a very high rate of urea breakdown and this rate may be too fast for long term in vitro studies of calcium carbonate precipitation. The genetically modified organisms Escherichia coli DH5α(pURE 14.8), Pseudomonas aeruginosa MJK1, and E. coli MJK2 were also measured in controlled batch experiments for urea breakdown. The rate of urea breakdown was modeled using a Michaelis-Menton equation; the observed V[subscript: max] was determined using urea, ammonium, and cell concentrations. The relative level of activity in descending order was found to be Sporosarcina pasteurii, E. coli MJK2, E. coli DH5α(pURE 14.8), and Pseudomonas aeruginosa MJK1. This ureolytic activity of bacteria may aid in a better understanding of how to produce an effective calcium carbonate precipitation result when introducing urea into a contaminated aquifer.
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