Honors College Thesis
 

Culturable Wood Endophytes of Populus trichocarpa

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  • This study is believed to be the first of its kind to characterize culturable fungal endophytes from the wood of Populus trichocarpa, and to examine the potential of inducible resistance and secondary chemistry as specific host factors that shape fungal endophytic communities in wood. To do so, more than seventy endophytes were isolated and cultured from woody tissue samples of Populus trichocarpa and identified through the partial amplification of the internal transcribed spacer (ITS) and the 28S nuc rDNA (LSU) sequences. Phylogenetic trees were constructed from the more conserved LSU region to visually represent the evolutionary relationships of the community of culturable fungal wood endophytes. The isolation frequency and species richness of these endophytes were determined to assess how they might be affected by the canker severity score and S/G lignin ratio of their host. Results suggest that canker severity score is likely a significant host factor involved in shaping the fungal wood endophyte community. However, further studies are needed. In contrast, the S/G lignin ratio does not appear to be a statistically significant factor in shaping the fungal wood endophyte community.
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