- Purpose: The purposes of this study are to determine the within subject and between subject effects of play markings, gross motor toys, and a larger play space on the physical activity of children with and without disabilities. Method: A group comparison design was implemented and a total of 12 children participated in a weekly inclusive playgroup. Nine of the children were considered to be without a disability, while 3 children were diagnosed with varying disabilities. The children participated in two phases, A and B, with the A phase consisting of stationary toys and a smaller play space and the B phase encouraging physical activity with play markings, gross motor toys, and a larger play space. Weekly playgroup sessions were video recorded using a modified Go-Pro and the movement of each child was tracked using momentary time sampling, with a 5-second interval. Results: Differences were visually observed between the A phase and B phase, suggesting that incorporating play markings, gross motor toys, and a larger play space has potential in increasing physical activity for both children with and without disabilities. However, differences in displacement were not found between children with and without disabilities, as the children with disabilities had a higher average displacement. Suggestions for future research are provided, including replication of the study with a larger sample size of children with and without disabilities and a narrower age range.