Honors College Thesis
 

Identification of Pseudomonas syringae genes required for perception of host-derived virulence-inducing metabolites

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https://ir.library.oregonstate.edu/concern/honors_college_theses/8336h7272

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  • Pseudomonas syringae is a Gram-negative bacterium that can infect a variety of important crops. Infection by P. syringae is dependent on a type III secretion system (T3SS) that delivers effector proteins to plant cells to suppress host defenses. Previous work has shown that, in the presence of simple sugars, specific plant-derived metabolites are potent inducers of genes encoding the T3SS. However, how P. syringae perceives and responds to these host-derived signals to deploy its T3SS is largely unknown. In this study, we performed a transposon mutagenesis screen of DC3000 to identify mutants that lack perception of fructose and aspartic acid and consequently have reduced expression of T3SS-associated genes. We identified transposon insertion sites in 200 confirmed loss-of-response mutants using Illumina whole-genome sequencing. 120 genes coding for proteins involved in signal transduction, transcription regulation, transportation, metabolism, and motility were identified as candidates for involvement in the perception of host-derived metabolites and induction of the T3SS. Characterization of these genes will provide insights into the mechanisms by which P. syringae regulates virulence in response to host signals.We functionally characterized one screen-identified gene, setA, and demonstrated that it is required for avrRpm1 expression in response to multiple simple sugars.
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  • Supported by funding from SURE Science.
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  • 2019-05-18 to 2021-06-18
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