Exploring Chromodomain Genes in the Fungus Fusarium graminearum Through Targeted Genetic Manipulation

Honors College Thesis

Public Deposited

Citeable URL: https://ir.library.oregonstate.edu/concern/honors_college_theses/8623j0508

Descriptions

Attribute Name	Values
Creator	Peters, Alec J.
Abstract	DNA segments in the fungus Fusarium graminearum were identified as possible genes encoding for chromodomain proteins (CDPs), and targeted for genetic manipulation. Targeted single-gene deletion strains, as wells as GFP-S tag fusion strains, were generated from wildtype F. graminearum protoplasts, and verified strains were screened for phenotypic differences, such as changes in growth, appearance, and levels of certain histone marks in the genome. It was found that the FgCdp6 gene is required for normal growth and appearance of F. graminearum, and its protein product localizes to nuclei of asexual spores. Further studies including western blots suggested that the CDP encoded by the FgCdp6 gene is a homolog of the yeast protein Eaf3, which is involved in both histone acetylation and deacetylation complexes.
Resource Type	Presentation Honors College Thesis
Date Available	2014-07-22T15:53:28+00:00
Date Issued	2013-11-20
Degree Level	Bachelor's
Degree Name	Honors Bachelor of Science (H.B.S.)
Degree Field	BioResource Research
Degree Grantor	Oregon State University
Commencement Year	2013
Advisor	Freitag, Michael
Non-Academic Affiliation	Oregon State University. Honors College Oregon State University. Bioresource Research
Rights Statement	In Copyright
Publisher	Oregon State University
Peer Reviewed	No
Language	English [eng]
Replaces	http://hdl.handle.net/1957/50651

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Thumbnail	Title	Date Uploaded	Visibility	Actions
	Alec Peters_Thesis_BRR_Formatted.pdf	07/26/2017	Public
	Alec Peters_Thesis_Presentation_Final.pdf	07/26/2017	Public