- The ability of nanoparticles (NPs) to induce oxidative stress on organisms has been linked to NP cytotoxicity and genotoxicity for a variety of organisms. One proposed mechanism of this increased oxidative stress is the ability of NPs to generate reactive oxygen species (ROS). Due to the dynamic nature and small size of nanoparticles it is often very difficult to utilize their intrinsic properties to predict their toxicity, as is occasionally done for chemical compounds. This study is aimed at the development of a functional assay for prediction of NP toxicity utilizing both a modified, abiotic version of the dichlorofluorescein (DCF) assay and the traditional cellular DCF assay applied to whole organism zebrafish. It was found that through combination of the abiotic and biotic DCF assays lanthanide oxide (LnOx) NPs could be correctly flagged for further toxicological work up based on increased ROS generation. These flagged NPs were hypothesized to have increased toxicological effects on organisms. Collection of toxicity data from the Nanomaterial Biological Interactions (NBI) Knowledgebase (nbi.oregonstate.edu) confirmed that three out of the four LnOx NPs flagged by the DCF assays caused increased morbidity and mortality in the embryonic zebrafish model.
Key Words: lanthanide oxide, nanoparticles, redox characterization, toxicology, spectrophotometry, dichlorofluorescein, zebrafish