Honors College Thesis
 

Method validation of quantitative analysis of oxylipins in human plasma via mass spectrometry

Public Deposited

Downloadable Content

Download PDF
https://ir.library.oregonstate.edu/concern/honors_college_theses/g158br29d

Descriptions

Attribute NameValues
Creator
Abstract
  • Oxylipins are a class of bioactive lipid metabolites derived from polyunsaturated fatty acids (PUFAs) via enzymatic and non-enzymatic pathways. In order to elucidate their overall biological effects, the knowledge of the entire oxylipin profile is needed as oxylipins can have similar or opposing effects in regard to inflammatory events. Their role as inflammation mediators makes oxylipins a potential candidate for biomarkers in cardiovascular diseases and cancers. A sensitive and reproducible method is needed in order to quantify the oxylipin profile as these species are found at very low endogenous concentrations in plasma and other tissues. A multiple reaction monitoring method (MRM) using liquid chromatography tandem mass spectrometry (LC-MS/MS) was used to separate, detect, and quantify the oxylipins from a complex human plasma sample matrix. The goal of this project was to complete a thorough method validation using the intra- and interday accuracy and precision, matrix effect, and analyte recovery. The method was able to quantify 65 oxylipins and three PUFAs from the panel and was shown to be reproducible as most of the oxylipin species were accurate and precise at an 80% threshold. In comparison to other published studies, this is the one of largest oxylipin panels to be quantified with 98% success in regard to targeted compounds. Overall, this analytical method allows for sensitive and accurate quantitative analysis of both pro- and anti-inflammatory lipid mediators that will be able to create a comprehensive oxylipin profile applicable to many human diseases involving inflammation. The next steps would be to continue to increase sensitivity using more sensitive mass spectrometry platforms and to validate the usefulness as biomarkers using clinical plasma samples and complimentary assays.
  • Key Words: oxylipins, mass spectrometry, analytical chemistry, biomarker
License
Resource Type
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Committee Member
Non-Academic Affiliation
Rights Statement
Publisher
Peer Reviewed
Language

Relationships

Parents:

This work has no parents.

In Collection:

Items

Thumbnail Title Date Uploaded Visibility Actions
file details BloomMadelineB2021.pdf 2021-05-26 Public Download