Honors College Thesis
 

Exploring the toxigenicity and genetic similarity of Detroit Reservoir’s recurring cyanobacterial bloom in 2017 and 2018

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  • In order to understand the ability of Detroit Reservoir’s recurring cyanobacterial bloom to produce toxins, and determine the genetic similarity of the bloom from year to year, environmental samples were taken from the Detroit Reservoir bloom biomass in the summers of 2017 and 2018. DNA from these samples was sequenced, assembled, binned, error corrected, and annotated. Both samples were aligned to each other to determine their genetic similarity between the years, and both samples were aligned individually to a group of known cylindrospermopsin-producing toxin genes from a well-studied Aphanizomenon sp. 10E6 genome to determine their toxigenicity. The 2018 sample genome was completed and sent to the NCBI for confirmation and annotation. After alignment, the 2017 and 2018 samples were found to be 99.99% identical organisms, and both were found to contain toxin-producing genes on plasmids within their larger genome structures.
  • Key Words: Cyanobacterial harmful algal blooms, Genome assembly, annotation, alignment
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