Honors College Thesis


Hypothalamic Leptin Gene Therapy Does Not Prevent Ovariectomy-Induced Bone Loss in Rats Public Deposited

Downloadable Content

Download PDF


Attribute NameValues
  • Post-menopausal women are at an increased risk for osteoporosis due to increased bone resorption associated with estrogen deficiency. Subcutaneous administration of leptin, a hormone produced primarily by adipocytes, attenuates bone loss in the ovariectomized (ovx) rat, an animal model for post-menopausal bone loss. Leptin has both direct peripheral and indirect central nervous system-mediated effects on bone. In the present study we evaluated the efficacy of increased central leptin, via hypothalamic leptin gene therapy, in preventing bone loss in ovx Sprague Dawley rats. Ovx rats were injected in the hypothalamus with either rAAV-lep (recombinant adeno-associated virus encoding leptin) or rAAV-GFP (control vector encoding green fluorescent protein) and maintained for ten weeks. As expected, bone volume/tissue volume in the lumbar vertebra and bone area/tissue area in the proximal tibia were lower in ovx compared to intact rats. Differences in osteoclast perimeter, an index of bone resorption, were not detected with treatment. Osteoblast perimeter, an index of bone formation, was higher in ovx rAAV-lep rats compared to intact rats. Differences between rAAV-lep and rAAV-GFP-treated rats were not detected for any endpoints evaluated. The results suggest that increasing central leptin has no positive or negative effect on bone mass or architecture in ovx rats.
Resource Type
Date Available
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Committee Member
Non-Academic Affiliation
Rights Statement
Peer Reviewed
Additional Information
  • description.provenance : Submitted by Heather Boren (heather.boren@oregonstate.edu) on 2010-07-06T20:12:19ZNo. of bitstreams: 1Melanie Jackson Thesis.pdf: 534902 bytes, checksum: 4400126dd885ff3d0bc369c2e6ec143b (MD5)
  • description.provenance : Made available in DSpace on 2010-08-13T18:02:33Z (GMT). No. of bitstreams: 1Melanie Jackson Thesis.pdf: 534902 bytes, checksum: 4400126dd885ff3d0bc369c2e6ec143b (MD5)



This work has no parents.

In Collection: