- Dissolved Organic Carbon (DOC) originates from organic matter. Climate and vegetation have been found to alter DOC production. The goal of this study was to determine where the differences in DOC production originate from. Mineral soil and substrate including maple leaves, needles, old wood, and sticks were collected from the HJ Andrews Research Forest in Oregon. A sample of Oa and Oe horizon soil was taken from the McDonald Dunn Research Forest. Soil columns were constructed, treatments included each substrate type with and without mineral soil. Half of the columns were kept at 20°C and half at 5°C. Columns were watered with 15 mL of a rainwater solution every 3 days for 75 days. DOC concentration was analyzed with Shimadzu TOC-V. The soil columns were sealed at regular intervals and respiration was measured with a Picarro Cavity Ring Spectrometer. There was little change in production of DOC as a result of mineral soil addition or temperature difference. However, there was an increase in respiration at the higher temperature. There was a difference in DOC production as a result of substrate quality with the high-quality substrates producing more DOC than the lower quality substrates, a similar relationship was seen with respiration.