Honors College Thesis

 

Comparative lipid profiling of human stratum corneum between atopic dermatitis and healthy subjects Public Deposited

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Access restricted, at author's request, from May 20, 2016 - May 20, 2018.

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  • Dermatological issues are a widespread problem in humans that are often overlooked. In our present study, we have isolated lipids from de-identified epidermal skin tape samples from both Atopic Dermatitis and healthy individuals using a high efficiency one-step extraction method, which were subsequently analyzed using modified Liquid chromatography–mass spectrometry (LC-MS). A study was performed on both an original set of samples and follow up samples from patients revisiting the clinic several months later. From our initial study, we have observed a general trend of increased triglycerides, CER[A], CER[N], and cholesterol-3-sulfate, decreased free fatty acids of specific chain length, and both increased and decreased CER[EO] subclass ceramides. In the follow up study, there was a general trend of unaltered cholesterol and triglyceride levels, normalized cholesterol-3-sulfate, decreased free fatty acids, CER[EO] and CER[N] subclass ceramides, and decreased/normalized CER[A] subclass ceramides. All of these changes in lipid composition are likely affecting the AD status of the individuals and influencing their barrier functions since lipids play a major role in the formation and maintenance of epidermal barrier of the skin. Altered lipid composition is likely a contributing factor towards impaired skin barrier functions and the manifestation of symptoms related to skin problems that are likely life long issues in AD subjects.
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  • 2017-07-27 to 2018-05-20

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