Honors College Thesis
 

Dissecting DNA Damage Responses in Arabidopsis: A High-Throughput Sequencing Approach

Público Deposited

Contenido Descargable

Descargar PDF
https://ir.library.oregonstate.edu/concern/honors_college_theses/mw22v744q

Descriptions

Attribute NameValues
Creator
Abstract
  • A unique, permanent root-growth arrest phenotype was observed in UV-B irradiated Arabidopsis thaliana roots lacking the DNA damage response kinase ATR. Segregation analysis suggests the dependence of this phenotype on another, unidentified gene, termed ursu. High-throughput sequencing technologies were used to map and identify the causal ursu mutation. Illumina HiSeq 2000 short sequence reads were analyzed using bioinformatics command line tools for Next-Generation Mapping (NGM) and Mapping and Alignment with Short Sequences (MASS) programs. Several putative candidate genes were identified, including auxin response factor 13 (arf13) and resistance to Peronospora parasitica protein 8 (rpp8). Sanger sequencing verified the presence of a single nucleotide polymorphism (SNP) and a frameshift mutation caused by a 26-bp deletion in arf13 in ursu atr plants. Although additional sequencing and complementation tests are still necessary, a causative mutation in arf13 could provide clues linking the DNA damage response kinase ATR with the key growth and development phytohormone auxin.
License
Resource Type
Fecha Disponible
Fecha de Emisión
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Non-Academic Affiliation
Declaración de derechos
Publisher
Peer Reviewed
Language
Replaces

Relaciones

Parents:

This work has no parents.

En Collection:

Elementos

Miniatura Título Fecha de subida Visibilidad Acciones
file details Brar_UHC_Thesis_2013.pdf 2017-07-29 Público Descargar