Honors College Thesis

 

Comparison of three diagnostic assays in the detection of Pseudoloma neurophilia in zebrafish Public Deposited

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https://ir.library.oregonstate.edu/concern/honors_college_theses/n009w818g

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  • As the zebrafish (Danio rerio) becomes increasingly popular as a biomedical model for various laboratory studies, the need for efficient and accurate diagnostic assays for the pathogens that infect it has grown. The most common pathogen infecting laboratory zebrafish is Pseudoloma neurophilia, a microsporidium that has been known to cause both overt and subclinical chronic disease. This study was performed in an effort to inform the Oregon Veterinary Diagnostic Service’s (OVDL) implementation of a broad panel of tests for zebrafish pathogens with the intent of having all tests ran in a standardized format for extraction from whole fish preparations. Two different populations of fish were sampled and divided into three groups that were tested for P. neurophilia by whole body qPCR, central nervous system qPCR, and histology. After analysis via Chi-square tests with Yates correction, it was determined that the detection of P. neurophilia is enhanced with two histological sections and qPCR tests. Key Words: Pseudoloma neurophilia, Danio rerio
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