Honors College Thesis

 

Supplemental Description of Myxobolus squamalis (Myxozoa) & Epidemiology of M. squamalis at Two Oregon Hatcheries Public

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  • Myxobolus squamalis is a Myxozoan parasite of salmonids, which contributes to lowered fitness of hatchery and wild fish in the Pacific Northwest of North America. The only GenBank DNA sequence of M. squamalis is from Oncorhynchus tschawytscha (Chinook), but is not linked to a published morphological description. There is a range of salmonid Myxozoans with similar morphology to M. squamalis and we suspect that some “M. squamalis” observations from different host species might be invalid. We provide a clear re-description of M. squamalis from its type host, O. mykiss (rainbow trout and steelhead). Our results confirm the morphological and morphometric data of the original description, which we supplement with a SSU rRNA gene sequence. Based on this sequence, we developed a PCR assay for specific detection of M. squamalis DNA in environmental water samples. We used the assay to compare parasite levels in the water influent and effluent from two hatcheries that rear different salmon and trout species. Our approach allows for early M. squamalis detection, infection level estimates, and estimates for environmental impacts based upon spore release. Early detection of Myxozoan infections is crucial to hatchery and wild fish management, as there is no documented treatment for infected fish.
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  • description.provenance : Approved for entry into archive by Sue Kunda(sue.kunda@oregonstate.edu) on 2012-08-07T18:08:19Z (GMT) No. of bitstreams: 1Polley, Tamsen_UHC Thesis (2012).pdf: 2059905 bytes, checksum: 08914e3d63ce8fe554c09abc242fa467 (MD5)
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