- Electrochemical sensing provides a quantitative alternative to colorimetric reactions within paper-based devices. The paper-based devices were fabricated by screen printing the conductive and reference inks onto porous substrates that were evaluated using cyclic voltammetry. Devices were evaluated based on the magnitude of the average anodic peak height, which correlates with a higher signal received from the electrode system, and the coefficient of variation, which correlates with the reproducibility of the devices. The first set of studies compared two conductive carbon ink solvents, two silver ink solvents, and two substrates. The Whatman cellulose, the organic-based solvent carbon ink, and the organic-based solvent silver ink was the most optimal combination with the second highest magnitude of the average anodic peak height (5.61µA) with the smallest coefficient of variation (4.47%). A shelf life study was conducted on this ink and material combination to determine the effect that storage time had on the electrode system. Even though there was not a statistically significant change in the signal produced and the time after fabrication, due to the limited number of replications on the later days, a shelf life of seven days was supported.