Developing a protocol for producing multiple BYDV resistant wheat genotypes from a single transformational event Public

http://ir.library.oregonstate.edu/concern/honors_college_theses/q811km67j

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  • Barley yellow dwarf (BYD) is a damaging luteovirus disease responsible for reduced tillering, delayed heading, sterility, and stunting in many cereals worldwide. Well-characterized resistance genes to barley yellow dwarf viruses (BYDV) are scarce and genes conferring tolerance to BYDV are limited in wheat. In an attempt to generate plants with resistance to BYDV, callus of common wheat (Triticum aestivum) was bombarded with particles coated with a transgene designed to produce hairpin RNA containing BYDV sequences to induce post transcriptional gene silencing. From the 27 putative transgenic lines generated, both PCR and herbicide screening revealed that none were positive for the BYDV transgene. The inability to acquire transgenic plants from the procedures accentuates the difficulty of wheat transformation. The lack of successful transformation may have been due to the low number of calli bombarded in this study; nonetheless, the likelihood of obtaining transgenic wheat plants would improve by increasing the amount of calli used to generate transformants.
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