Honors College Thesis


Investigation of the effects of cryopreservation on cellular viability and mechanical integrity of the intervertebral disc Public Deposited

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  • Degeneration of the intervertebral disc (IVD) is a phenomenon that has yet to be adequately addressed. Treatment options remain limited and incomplete. The ability to effectively cryopreserve IVDs would allow for better allograft transplantations and storage of samples for research purposes. This study aimed to assess how cryopreservation with different pre-freeze incubation times and the inclusion of PrimeGrowth in the protocol would affect the cellular viability and mechanical integrity of the IVD. Whole bovine caudal IVDs (n = 18) were harvested and processed using various pre-freeze incubation times of 2-hours, 12-hours, and 20-hours. After storage in a -80oC freezer, the cryopreserved IVDs were quickly thawed for mechanical compression testing and cellular viability imaging. The low-strain elastic modulus, high-strain elastic modulus, and viability were calculated and compared to fresh control, negative control, and non-PrimeGrowth treated groups. There was no statistical difference between treatment groups for the low-strain or high-strain elastic modulus. Groups with 2-hour and 12-hour incubation times showed no difference from the negative control in viability. The 20-hour incubation group showed the highest cell viability among the treated groups. The non-PrimeGrowth treated group showed lower viability than the 20-hour group but higher than 2-hour and 12-hour groups.
  • Intervertebral Disc (IVD), Cryopreservation, Biomechanics, Back Pain, PrimeGrowth
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