- High Performance Liquid Chromatography-Mass Spectrometry coupled with filtration, centrifugation, and other common sample preparation techniques offers a rapid and robust method for separating and quantifying propofol, a commonly used anesthetic, from human blood and its synthetic analogs. Method development is important for not only optimizing separation and quantification for a sample in a given matrix, but also for doing so on a specific instrument. The first part of thesis focuses on this method development by determining or offering the experimental framework to determine optimal detection wavelength, flowrate, instrument (i.e. nano versus conventional HPLC), and mobile phase. The second part applies this technique to assess if alcoholic liver disease affects the minimum effective dose, recovery time, and depth of sedation in intensive care unit patients receiving propofol to be intubated due to alcohol withdrawal.