Investigation of autophagy-assisted cell death in response to the cancer cell toxin coibamide A

Honors College Thesis

Public Deposited

Citeable URL: https://ir.library.oregonstate.edu/concern/honors_college_theses/12579x96m

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Attribute Name	Values
Creator	Tan, Michelle
Abstract	Autophagy, a homeostatic degradation pathway, has a role in promoting cell survival under stress conditions, but can also promote cell death under conditions of sustained stress. This project investigates the cytotoxic potential of coibamide A and other natural products in autophagy-deficient and wild-type mouse embryonic fibroblasts (MEFs). Wild-type MEFs were more vulnerable to coibamide A than cells lacking autophagy-related protein 5 (Atg5). Through cell viability assays and immunoblotting for markers of autophagy, it was found that restoration of expression of Atg5, a vital autophagy-related protein, in an autophagy-null background was able to partially restore the response characterized in wild-type MEFs. These results lend support to the existence of crosstalk between autophagy and apoptosis, and suggest coibamide A as a compound with characteristics that may utilize autophagy for pro-death signaling.
License	All rights reserved
Resource Type	Honors College Thesis
Date Available	2015-09-01T20:01:36+00:00
Date Issued	2015-08-20
Degree Level	Bachelor's
Degree Name	Honors Bachelor of Science (H.B.S.)
Degree Field	Biochemistry and Biophysics
Degree Grantor	Oregon State University
Commencement Year	2016
Advisor	Ishmael, Jane E.
Non-Academic Affiliation	Oregon State University. Honors College
Rights Statement	In Copyright
Publisher	Oregon State University
Peer Reviewed	No
Language	English [eng]
Replaces	http://hdl.handle.net/1957/57049

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