Undergraduate Thesis Or Project
 

Corey,K Infection 11-14.pptx

公开 Deposited

可下载的内容

下载文件
https://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/1j92gc98d

Descriptions

Attribute NameValues
Creator
Abstract
  • Symbiotic relationships between cnidarians and dinoflagellates within the genus Symbiodinium are critically important for the success of coral reefs. These symbionts provide their hosts with photosynthetically-fixed carbon in exchange for nutrients and shelter. Symbiodinium is a phylogenetically diverse genus that is often typed by sequencing the ribosomal internal transcribed spacer (ITS) region. However, this approach may miss fine-scale genetic differences among the types, which may have large functional importance. Here we examined differences in host specificity by inoculating aposymbiotic individuals of the sea anemone Aiptasia sp. with six different cultured strains of type “B1” Symbiodinium isolated from different cnidarian hosts. Infection was tracked over time using image analysis of Symbiodinium autofluorescence as a rapid and non-destructive proxy for cell counting. There were significant differences in infection rates among the different Symbiodinium strains compared to a B1 strain initially isolated from Aiptasia. Specifically, a strain cultured from the Caribbean octocoral Antillogorgia elisabethae had significantly lower infectivity compared to the native strain. This corresponded to larger genetic distance between these two cultures as diagnosed by restriction site associated DNA (RAD) sequencing of SNPs compared to other B1 cultures that infected at similar rates. This indicates high specificity between Aiptasia and Symbiodinium.
  • Keywords: Symbiosis, Infection, Aiptasia, Auto Fluorescence, Coral Bleaching, Symbiodinium
权利声明

关联

Parents:

单件

缩略图 标题 上传日期 公开度 行动
file details Corey,K Infection 11-14.pptx 2017-11-09 公开 下载