Method development for extraction and purification of dermal RNA from FVB/N mice treated with environmental PAH mixtures Public Deposited

http://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/1n79h6268

August 2010

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  • Epidermal RNA samples from FVB/N mice treated with PAH standards and environmental PAH mixtures were harvested and purified using Trizol extraction and RNeasy mini prep kit purification. Epidermal RNA integrity was low (RIN mean 3.5), indicating significant RNA degradation and the need to modify handling, collection, and processing of skin to limit degradation. Potential sources of RNA degrdation include RNase activity, both endogenous and exogenous, thermal degradation during heatinduced epidermal-dermal seperation, and contamination of stock solutions. Testing of the modified method produced dermal RNA samples with acceptable RIN integrity levels (RIN mean 7.6). Variations of the modified method were performed and compared to one another for method optimization.
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  • description.provenance : Made available in DSpace on 2010-09-14T16:28:09Z (GMT). No. of bitstreams: 2 Larkin_seminar presentation-1.pdf: 1877964 bytes, checksum: 3735e98f1fb2c414d1ff994120658b66 (MD5) Larkin_BRR_final thesis.pdf: 936734 bytes, checksum: 873f2836be2ad48980acf0a6f7f47191 (MD5)
  • description.provenance : Submitted by Wanda Crannell (crannelw@hort.oregonstate.edu) on 2010-09-14T16:28:08Z No. of bitstreams: 2 Larkin_seminar presentation-1.pdf: 1877964 bytes, checksum: 3735e98f1fb2c414d1ff994120658b66 (MD5) Larkin_BRR_final thesis.pdf: 936734 bytes, checksum: 873f2836be2ad48980acf0a6f7f47191 (MD5)

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