Honors College Thesis
 

Investigating the Potential for Cryopreservation of Human Granulocytes with Concentrated Glycerol

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  • Granulocytes are a class of white blood cells essential to immune function. Granulocyte transfusions hold promise to treat many conditions, but the short-shelf life of granulocytes makes transfusions infeasible without a cell preservation method. The purpose of this study was to investigate the potential for cryopreservation of granulocytes using 30% glycerol. Recently reported permeability data was used to design two different methods for addition and removal of glycerol: a fast method that is predicted to keep cell volumes between 80% and 150% of the isotonic volume and a slow method that is predicted to keep cell volumes between 80% and 115% of the isotonic volume. The fast method was designed to minimize glycerol toxicity, while the slow method was designed to minimize osmotic damage. The fast method resulted in cell recoveries of 31% ± 9% and 11% ± 3% before and after freezing, respectively, whereas the slow method resulted in even lower cell recoveries of 5% ± 2% and 4% ± 2%. The reduced cell recovery for the slow method is consistent with an increase in damage as a result of glycerol toxicity rather than osmotic damage. These results suggest that cryopreservation of granulocytes in concentrated glycerol is not feasible.
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file details MossAldenC2017.pdf 2017-07-29 Pubblico Scaricare