Molecular Dynamics on REX-GECO1 Reveal Structural Features Governing Fluorescence

Undergraduate Thesis Or Project

Public Deposited

Citeable URL: https://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/2z10wx846

Descriptions

Attribute Name	Values
Creator	Rozanov, Nikita
Abstract	Fluorescent proteins have emerged as an essential toolset for bioimaging, creating a demand for engineering proteins with new and improved fluorescent properties. In this thesis, I explore the atomistic structure of REX-GECO1, a newly engineered protein biosensor that has unique optical properties. Since this protein has no available crystal structure, understanding the relationship between its structure and properties is difficult. To overcome this challenge, I use molecular dynamics (MD) simulations to predict the protein's structure and use this information to identify structural features that influence fluorescence. Moreover, I use the simulations to obtain thermodynamic information that provides further detail about the protein. These findings will be useful for understanding data obtained from ongoing ultrafast spectroscopic studies.
Resource Type	Undergraduate Thesis
Date Issued	2018-05-29
Degree Level	Bachelor's
Degree Name	Bachelor of Science (B.S.)
Degree Field	Physics
Degree Grantor	Oregon State University
Commencement Year	2019
Advisor	Fang, Chong
Academic Affiliation	Physics
Rights Statement	In Copyright
Publisher	Oregon State University
Peer Reviewed	No
Language	English [eng]

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