Undergraduate Thesis Or Project
 

Final_Seminar_Jose_Solis.pdf

Público Deposited

Contenido Descargable

Descargar PDF
https://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/3197xp15g

Descriptions

Attribute NameValues
Creator
Abstract
  • Vibrio cholerae a major health problem in developing countries because of its ability to cause the disease cholera in humans. As V. cholerae bacteria are common inhabitants of temperate marine and brackish water environments worldwide, they are subject to intense top-down pressure by amoebae, which can affect their survival and replication in natural reservoirs. In these environments, interactions with amoebae, single celled eukaryotic predators of bacteria, are inevitable. This study examined the interactions between a model amoeboid predator, Acanthamoeba castellanii, and diverse strains of V. cholerae to better understand the adaptations of these bacteria to eukaryotic grazing pressure over 48 hours. Our analysis showed that V. cholerae TP (non-pathogenic GFP variant) and AR4100 (pathogenic GFP variant) increased growth (TP growth difference: 4.29-fold, AR4100 growth difference: 4.67- fold) when co-cultured with A. castellanii in comparison to E. coli co-culture, while SIO (non- pathogenic GFP variant) had no growth difference (SIO growth difference: -1.05-fold). In general, V. cholerae isolates induced cyst formation in amoebae within 48 hours and remained viable after ingestion. These findings suggest that complex predator-prey relations may ultimately impact the survival of strains of these potential pathogens in the environmental reservoirs in significantly different ways, changing the population structure of these organisms in their communities.
  • Keywords: Bacterial viability, Top down pressure, Predation prey interactions, Biofilm formation, Amoeba phenotypic changes, Pathogenesis
Declaración de derechos

Relaciones

Parents:

Elementos

Miniatura Título Fecha de subida Visibilidad Acciones
file details Final_Seminar_Jose_Solis.pdf 2017-07-27 Público Descargar