Undergraduate Thesis Or Project

 

DNA mismatch repair and 0[superscript 6]-methylguanine toxicity : Testing the futile repair cycle hypothesis Public Deposited

Analytics

Downloadable Content

Download PDF
https://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/3x816s00q

Descriptions

Attribute NameValues
Creator
Abstract
  • Cell lines deficient in certain genes of the DNA mismatch repair (MMR) system show a marked resistance to the cell killing or apoptotic effects of DNA alkylating agents. The molecular mechanism for this resistance is not yet understood, but is currently of great interest because of the recent use of these agents in cancer chemotherapy. Of particular interest is the role of the primary mutagenic base-adduct, 06-methylguanine (06-meG), in this MMR-dependent resistance. In this paper, an assay for testing one of the hypotheses for the MMR-dependent resistance to alkylating agents (the futile repair cycle) is described and the progress towards performing such an assay is reported. Specifically, single-stranded phage DNA was prepared with less than 5% double-stranded DNA contaminant, fl phage gene II-protein was purified with a specific activity of approximately 5% of the published value using an abbreviated protocol, and MMRcompetent HeLa cell nuclear extract was prepared with an in vitro repair efficiency of 30%. Also, the possibility of improving in vitro repair assays by using alkaline phosphatase in DNA substrate preparation is investigated.
License
Resource Type
Date Available
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Non-Academic Affiliation
Rights Statement
Publisher
Peer Reviewed
Language
File Format
File Extent
  • 2390576 bytes
Digitization Specifications
  • Master files scanned at 600 ppi (256 Grayscale) using Capture Perfect 3.0 on a Canon DR-9080C in TIF format. PDF derivative scanned at 300 ppi (256 Grayscale), using Capture Perfect 3.0, on a Canon DR-9080C. CVista PdfCompressor 3.1 was used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Approved for entry into archive by Linda Kathman(linda.kathman@oregonstate.edu) on 2007-12-11T16:06:52Z (GMT) No. of bitstreams: 1Brodeur_T.pdf: 2390576 bytes, checksum: 3cf4969c454b30554d6c7f901eda8c2b (MD5)
  • description.provenance : Submitted by Sara MIntonye (smscanner@gmail.com) on 2007-12-06T22:41:43ZNo. of bitstreams: 1Brodeur_T.pdf: 2390576 bytes, checksum: 3cf4969c454b30554d6c7f901eda8c2b (MD5)
  • description.provenance : Made available in DSpace on 2007-12-11T16:06:52Z (GMT). No. of bitstreams: 1Brodeur_T.pdf: 2390576 bytes, checksum: 3cf4969c454b30554d6c7f901eda8c2b (MD5)

Relationships

Parents:
In Collection:

Items

Thumbnail Title Date Uploaded Visibility Actions
Default Brodeur_T.pdf 2017-10-27 Public