Identification of potential new biomarkers of oxygenated polycyclic aromatic hydrocarbon (OPAH) exposures in zebrafish. Public Deposited

http://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/41687k158

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  • Oxygenated polycyclic aromatic hydrocarbons (OPAHs) are a class of environmentally prevalent compounds with little known information on their effect in vivo. In a recent study conducted using zebrafish as a model, 38 OPAHs were screened for toxicity. This investigation focuses on two of these OPAHs, 7,12-B[a]AQ and BEZO. Both 7,12-B[a]AQ and BEZO show AHR2-dependent toxicity, however only 7,12-B[a]AQ consistently and significantly induces expression of cyp1a protein. Cyp1a is a diverse xenobiotic metabolizing protein that is used as a biomarker for many AHR-dependent toxicity studies in vertebrates. As 7,12-B[a]AQ and BEZO differentially express cyp1a, this study focused on four genes, ctsl.1, wfikkn1, ponzr3, and ponzr4, identified by RNA-sequencing as potential alternative biomarkers for AHR-dependent OPAH toxicity. A time point analysis of gene expression of ctsl.1 and wfikkn1 concluded that the stage of development is important in selecting alternative biomarkers. Preliminary localization of all four gene mRNA transcripts using in situ hybridization suggests that expression is sensitive to both stage of development and specific OPAH exposure. Investigating the candidacy of potential alternative biomarkers for AHR-dependent OPAH toxicity would help determine the pathway of toxicity of OPAHs in any environmental sample.
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