Honors College Thesis
 

Interaction of the Respiratory Syncytial Virus Matrix Protein and the Cellular Adaptor Protein Complex 3µ Subunit

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https://ir.library.oregonstate.edu/concern/honors_college_theses/5h73pz00k

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  • Respiratory Syncytial Virus (RSV) is a leading cause of bronchopneumonia in infants and elderly. Knowledge of viral and host protein interactions is important for better understanding of the viral pathogenesis and may lead to development of novel therapeutic drugs. Here, we show that RSV Matrix (M) protein interacts with cellular adaptor protein complex (AP) 3 and its medium (µ) subunit (AP3µ1). A yeast two-hybrid assay indicated a novel protein-protein interaction that was then further confirmed in a mammalian system by colocalization between the RSV M and AP3µ1 proteins in a cytoplasmic defined region via Confocal Laser Scanning Microscopy (CLSM) analysis. Further evidence of this novel interaction was indicated via the presence of a known adaptor protein µ subunit sorting signal sequence, YXXL, which is conserved across various animal RSV M proteins. Subsequent studies also showed a specific up-regulation in the amount of AP3µ1 protein found in the cell during RSV infection, while corresponding subunits of the AP3 complex were unaffected. The interaction of AP3µ1 with RSV M represents a critical insight into the life cycle of this important virus protein.
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