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Expression of Deer Adenovirus Spike Protein

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https://ir.library.oregonstate.edu/concern/defaults/6108vc840

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  • Research Outline •Amplify the coding region of the spike protein by PCR using primer flanking the S protein coding region •Clone the coding region into PCR-TOPO 2.1 cloning vector •Re-clone the coding region into PET6XHN-N vector •Induce the spike protein expression in BL-21 cells
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