Understanding Regulatory DNA in Plants Through Molecular Cloning and Transient Transformation Public Deposited

http://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/6682x576r

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  • Gene expression in plants (and other eukaryotes) is a very complex process. Gene expression is primarily controlled by proteins and their binding sites near the gene in the chromosomal DNA. More specifically, interactions among transcription factors (DNA-binding proteins) and cis-regulatory modules (CRMs-sets of binding sites) control the expression of nearby genes. The Fowler lab has been working towards understanding how CRMs work by using an in vivo quantitative method of examination. By establishing a plasmid construct enabled for Golden Gate Cloning with a fluorescent gene reporter, and a method to analyze the CRMs via biolistic transformation, further understanding of how these CRMs work alone or with different combinations can be achieved. Slight variation was found in quantitative data with biolistic transformations from day to day, but the method has promise as a quick and stable way of analyzing sequences of interest. Some validation for the method of examination was accomplished, but further research is needed to characterize the CRMs of interest.
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  • description.provenance : Made available in DSpace on 2016-09-08T20:57:07Z (GMT). No. of bitstreams: 4 license_rdf: 1223 bytes, checksum: d127a3413712d6c6e962d5d436c463fc (MD5) Thesis_Cesar Juarez_BRR.docx: 8813110 bytes, checksum: 1dd64c7c75c1b8aa5da4c01d77e1629d (MD5) Thesis_Cesar Juarez_BRR.pdf: 4970161 bytes, checksum: 4f9772e245aaef690d4c4ea188b68d16 (MD5) Seminar_BRR_Cesar Juarez.pptx: 55994483 bytes, checksum: 1083cf501be42921505c1cd2e4db655d (MD5)
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