Undergraduate Thesis Or Project
 

Understanding Regulatory DNA in Plants Through Molecular Cloning and Transient Transformation

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https://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/6682x576r

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  • Gene expression in plants (and other eukaryotes) is a very complex process. Gene expression is primarily controlled by proteins and their binding sites near the gene in the chromosomal DNA. More specifically, interactions among transcription factors (DNA-binding proteins) and cis-regulatory modules (CRMs-sets of binding sites) control the expression of nearby genes. The Fowler lab has been working towards understanding how CRMs work by using an in vivo quantitative method of examination. By establishing a plasmid construct enabled for Golden Gate Cloning with a fluorescent gene reporter, and a method to analyze the CRMs via biolistic transformation, further understanding of how these CRMs work alone or with different combinations can be achieved. Slight variation was found in quantitative data with biolistic transformations from day to day, but the method has promise as a quick and stable way of analyzing sequences of interest. Some validation for the method of examination was accomplished, but further research is needed to characterize the CRMs of interest.
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  • Agriculture and Food Research Initiative Grant no 2014-67013-21661 from the USDA National Institute of Food and Agriculture (Fowler lab support)Botany & Plant Pathology - Ernest and Pauline Jaworski Fund for Summer Research ExperiencesBioenergy Education Project Internship and Research
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