Functionalization of Surfaces with Nisin in a Polyethylene Oxide Brush Layer Public Deposited

http://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/6h440v57h

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  • Infections in hospitals account for over 100,000 deaths per year. These infections occur at the hospital from complications following bacterial adhesion to intravenous catheters, coronary stents and other implanted devices. Another common problem is protein adsorption to the surface of the device and subsequent blood clotting. Methods for combating these issues have been previously studied using antimicrobial coatings consisting of a tri‐block polymer with a hydrophobic base and two hydrophilic tails called Pluronic® F108 and an antimicrobial agent, nisin; however studies have not been conducted to determine how long such coatings are effective. Experiments were designed to test the long term efficacy of an F108 coated, nisin loaded surface for killing the Gram positive bacteria, Pediococcus pentosaceus. The overall experimentation, consisting of aging nisin‐coated microspheres for selected periods of time, followed by assaying bioactivity to determine nisin effectiveness, lasted 28 days, with samples taken from the microsphere suspensions once each week. These samples were plated on MRS agar plates and P. pentosaceus was allowed to grow for 48 hours, after which the colony forming units were counted for each sample. The F108‐coated, nisin loaded layers showed greater activity retention in comparison to layers prepared with nisin in the absence of F108, but only in the final week of the study.
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  • description.provenance : Submitted by Heather Boren (borenh) on 2008-06-19T18:24:16Z No. of bitstreams: 1 Ryder, Matt Honors Thesis Spring 2008.pdf: 6518438 bytes, checksum: 34a54262addb506b0807e351de000e0a (MD5)
  • description.provenance : Made available in DSpace on 2008-06-19T18:27:37Z (GMT). No. of bitstreams: 1 Ryder, Matt Honors Thesis Spring 2008.pdf: 6518438 bytes, checksum: 34a54262addb506b0807e351de000e0a (MD5)

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