Undergraduate Thesis Or Project


Analysis of boscalid resistance of isolates of Botrytis cinerea Pubblico Deposited

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  • Succinate dehydrogenase (Sdh) inhibitor fungicides, such as boscalid, are effective for the management of gray mold caused by the fungal pathogen Botrytis cinerea. Unfortunately, resistance to boscalid was common among isolates of the pathogen from small fruits grown in Oregon. Boscalid-resistance is commonly associated with mutations in Sdh, especially in subunit B. SdhB was sequenced from five boscalid-sensitive and five boscalid-resistant isolates of B. cinerea. A majority of the boscalid-resistant isolates had a single nucleotide polymorphism at codon 272 resulting in a substitution of histidine with arginine (H272R). The boscalid-sensitive isolates did not harbor this mutation. The transposons Boty and Flipper also were found within the genomes of the resistant strains, but not boscalid-sensitive isolates. Additionally, a pattern was found to exist between the transposons present within the strains and source location. Additional studies with a greater number of isolates are required to verify this correlation.
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