Exploring Chromodomain Genes in the Fungus Fusarium graminearum Through Targeted Genetic Manipulation Public

http://ir.library.oregonstate.edu/concern/honors_college_theses/70795953z

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  • DNA segments in the fungus Fusarium graminearum were identified as possible genes encoding for chromodomain proteins (CDPs), and targeted for genetic manipulation. Targeted single-gene deletion strains, as wells as GFP-S tag fusion strains, were generated from wildtype F. graminearum protoplasts, and verified strains were screened for phenotypic differences, such as changes in growth, appearance, and levels of certain histone marks in the genome. It was found that the FgCdp6 gene is required for normal growth and appearance of F. graminearum, and its protein product localizes to nuclei of asexual spores. Further studies including western blots suggested that the CDP encoded by the FgCdp6 gene is a homolog of the yeast protein Eaf3, which is involved in both histone acetylation and deacetylation complexes.
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