Characterization of the Sodium Transporting NADH:Quinone Oxidoreductase (NQR) in Vibrio cholerae Public Deposited

http://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/7m01bn63j

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Vibrio cholerae is the causative agent of cholera, a serious diarrheal disease in developing countries. V. cholerae has a unique redox driven respiration-linked sodium pump, Na⁺ translocating NADH:quinone oxidoreductase (NQR). Several reports previously showed that NQR plays an important role in virulence, metabolism, and sodium homeostasis of V. cholerae. This study aims to better understand the mechanisms of the effect of NQR on V. cholerae virulence, metabolism, and physiology. Analysis of the V. cholerae Δnqr mutant revealed that virulence gene expression is highly growth-phase dependent and virulence gene transcription is altered only at the early-log growth phase compared to the wild type. The Δnqr mutant also showed a broad range of metabolic defects, as assessed by a Phenotype Microarray™ (Biolog), suggesting the importance of NQR in the energy metabolism of V. cholerae. Furthermore, we also concluded that the effects of NQR on V. cholerae virulence gene expression were linked to respiration rather than sodium motive force. Taken together, these results suggest that NQR has broad growth dependent effects on V. cholerae metabolism and the observed changes in metabolism are linked to the observed changes in virulence gene expression following the loss of NQR.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Advisor
Non-Academic Affiliation
Keyword
Rights Statement
Funding Statement (additional comments about funding)
Language
Replaces
Additional Information
  • description.provenance : Approved for entry into archive by Deanne Bruner(deanne.bruner@oregonstate.edu) on 2013-07-10T17:56:20Z (GMT) No. of bitstreams: 1 Fassio UHC thesis.pdf: 1409630 bytes, checksum: 2553cc6372c04d62fe5ca301f0f5fa56 (MD5)
  • description.provenance : Made available in DSpace on 2013-07-10T17:56:20Z (GMT). No. of bitstreams: 1 Fassio UHC thesis.pdf: 1409630 bytes, checksum: 2553cc6372c04d62fe5ca301f0f5fa56 (MD5)
  • description.provenance : Submitted by Kassena Hillman (kassena.hillman@oregonstate.edu) on 2013-06-27T21:39:56Z No. of bitstreams: 1 Fassio UHC thesis.pdf: 1409630 bytes, checksum: 2553cc6372c04d62fe5ca301f0f5fa56 (MD5)

Relationships

In Administrative Set:
Last modified: 07/28/2017

Downloadable Content

Download PDF
Citations:

EndNote | Zotero | Mendeley

Items