Development of Fluorometric Assay for the Determination of β-glucosidase Activity in Brewing Yeasts Public

http://ir.library.oregonstate.edu/concern/honors_college_theses/7w62fd85z

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Glycosides are natural molecules found in many plants and hold potential to release new flavors and aromas into alcoholic beverages, particularly beer and wine. One way to free these flavors and aromas is by enzymatic hydrolysis catalyzed by βglucosidase. The activities of β-glucosidase are strain and species dependent in yeast as well as other organisms. This study focused on developing a rapid method for the determination of β-glucosidase activity of many brewing yeasts. Knowledge of βglucosidase activity would allow brewers to choose different yeasts depending on their desired effect. Through examining other studies a new method was developed using the substrate 4-MUG and beer-like growth conditions to measure intra-and extracellular yeast β-glucosidase activity. 18 yeasts were measured using this method, confirming previous findings that non-Saccharomyces yeasts tend to exhibit higher enzyme activity than Saccharomyces spp.
License
Resource Type
Date Available
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Non-Academic Affiliation
Rights Statement
Publisher
Peer Reviewed
Language
Replaces
Additional Information
  • description.provenance : Submitted by Andrew Molitor (molitora@onid.orst.edu) on 2015-06-05T21:17:21ZNo. of bitstreams: 2license_rdf: 1232 bytes, checksum: bb87e2fb4674c76d0d2e9ed07fbb9c86 (MD5)MolitorAndrewA2015.pdf: 768911 bytes, checksum: 3755cf018943450ff96d07351a4ced45 (MD5)
  • description.provenance : Made available in DSpace on 2015-06-08T17:22:14Z (GMT). No. of bitstreams: 2license_rdf: 1232 bytes, checksum: bb87e2fb4674c76d0d2e9ed07fbb9c86 (MD5)MolitorAndrewA2015.pdf: 768911 bytes, checksum: 3755cf018943450ff96d07351a4ced45 (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2015-06-08T17:22:14Z (GMT) No. of bitstreams: 2license_rdf: 1232 bytes, checksum: bb87e2fb4674c76d0d2e9ed07fbb9c86 (MD5)MolitorAndrewA2015.pdf: 768911 bytes, checksum: 3755cf018943450ff96d07351a4ced45 (MD5)

Relationships

Parents:

This work has no parents.

Last modified

Downloadable Content

Download PDF

Items