Anthocyanin and antioxidant analysis of sweet and tart cherry varieties of the Pacific Northwest Public Deposited

http://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/8336h653g

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  • Northwest cherry cultivars including Prunus avium `Bing', Prunus avium `Rainier', Prunus avium `Royal Anne' and Prunus cerasus `Montmorency' were separated into epidermal tissue, flesh and pit and cryogenically milled with liquid nitrogen. These samples as well as whole samples from each variety were extracted with acetone followed by a chloroform partition. The monomeric anthocyanin composition for each epidermal tissue sample was quantified using pH-differential methodology. Calculated (cyanidin-3-glucoside basis) levels were 130.0 ± 21 mg/100g of epidermal tissue for `Bing' cherries, 33.8 mg/100g for `Montmorency', 3.0 mg/100g ± 0.1 for `Rainier' and 10.5 ± 7 mg/100g for `Royal Anne'. HPLC, Mass Spectroscopy and spectral analyses, of Prunus avium L. indicated that >95% of the anthocyanin composition was comprised of cyanidin-3-glucoside, cyanidin-3-rutinoside and peonidin-3-rutinoside. Analyses for Prunus cerasus `Montmorency' found that >94% of its composition was cyanidin-3-glucosylrutinoside, cyanidin-3-rutinoside and peonidin-3-rutinoside. The antioxidant absorbing capacity of all varieties as well as separated samples of `Bing' and `Rainier' were measured using Oxygen free-Radical Absorbing Capacity (ORAL) and Ferric Reducing Antioxidant Power (FRAP) assays. Samples were measured for their free-radical binding capacity and compared using Trolox equivalents. `Bing' sample results were 30.1 ± 0.51mM for ORAC and 23.69 ± 0.44mM for FRAP, 23.07 ± 0.13mM and 26.09 ± 0.47mM FRAP for 'Montmorency', 18.28 ± 0.45mM ORAC and 12.69 ± 0.39mM FRAP for `Royal Anne' and 7.37 ± 0.08mM ORAC and 4.17 ± 0.43mM FRAP for `Rainier'.
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