Thesis_Cesar Juarez_BRR.docx

Undergraduate Thesis Or Project

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Citeable URL: https://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/9306t119d

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Attribute Name	Values
Creator	Juarez, Cesar
Abstract	Gene expression in plants (and other eukaryotes) is a very complex process. Gene expression is primarily controlled by proteins and their binding sites near the gene in the chromosomal DNA. More specifically, interactions among transcription factors (DNA-binding proteins) and cis-regulatory modules (CRMs-sets of binding sites) control the expression of nearby genes. The Fowler lab has been working towards understanding how CRMs work by using an in vivo quantitative method of examination. By establishing a plasmid construct enabled for Golden Gate Cloning with a fluorescent gene reporter, and a method to analyze the CRMs via biolistic transformation, further understanding of how these CRMs work alone or with different combinations can be achieved. Slight variation was found in quantitative data with biolistic transformations from day to day, but the method has promise as a quick and stable way of analyzing sequences of interest. Some validation for the method of examination was accomplished, but further research is needed to characterize the CRMs of interest. Keywords: Biolistic transformation, Fluorescent proteins, Genes, Maize, DNA
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