Undergraduate Thesis Or Project

Using Mass Spectrometry to Trace Polyphenols in Human1.pptx

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  • Diet History Questionnaires (DHQ) can only provide general information about a person’s nutritional intake. It is unable to cluster groups of people based on diets or lifestyles, nor identify specific foods that may play a role in disease prevention. The aim of this study was to validate the use of mass spectrometry as a method of assessing diet, in comparison to DHQs. Additionally, we used principal components analysis (PCA) to cluster subjects based on the concentrations of polyphenols. We compared values of polyphenols with values of DHQs. Sixty subjects’ urine were part of an on-going clinical study of Xanthohumol and Prevention of DNA Damage at the Linus Pauling Institute. DHQs were from the National Institute of Health (NIH) II version. Urine was analyzed using mass spectrometry coupled with an Ultra High Performance Liquid Chromatography (UHPLC). An algorithm was created to compare both the intensities of polyphenols and food groups from DHQ. Principal component analysis was used to analyze polyphenols separately from DHQs. In comparing mass spectrometry results with DHQ, the strongest correlation value (r >.60, P-value<0.05) was measured for secoisolariciresinol (SECO) and strawberry consumption. Using PCA, we found several polyphenols showed varying concentration between subjects; this validates mass spectrometry while showing the limited abilities of DHQs. Analyzing urine using mass spectrometry provided a more detailed analysis of diet when compared to the broad DHQs.
  • Keywords: Diet History Questionnaires, Polyphenols, Principal Component Analysis
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