Undergraduate Thesis Or Project

 

NEIL1 Deficiency Modulates the Effects of High Fat, High Carb, and Pro/Anti-oxidative Treatments in Mice Public Deposited

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  • Metabolic disorders affect much of the population, and it is widely believed that they have a genetic basis as well as being influenced by dietary factors. Recent studies have revealed an important role for the neil1 gene in the prevention of metabolic diseases. NEIL1 is a DNA Glycosylase that catalyzes the repair of DNA and mtDNA. We aimed to understand how a deletion in the neil1 gene in a mouse model would affect susceptibility to high fat and high carbohydrate diets. We also sought to establish a role for pro- and antioxidants in modulating disease risk. To accomplish this, Wild Type (WT) and neil1 Knockout (KO) mice were placed on either high fat or high carbohydrate diets. In a cohort of animals, the high fat diet was supplemented by the pro-oxidant KBrO3, or the antioxidant N acetyl cysteine. Under the oxidative stress of a high fat diet, the KO mice gained more weight than the WT mice. A similar difference was observed for the high carbohydrate treatment. The pro-oxidant KBrO3 seemed to mitigate the weight gain phenotype that was characteristic of a high fat diet, whereas the antioxidant NAC had no effect in both kinds of mice. At this point, the conclusion can be drawn that neil1-KO animals are more prone to the weight gain (and attendant effects of metabolic syndrome) induced by high fat and high carbohydrate diets.
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  • Center for Research on Occupational and Environmental Toxicology
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