NEIL1 Deficiency Modulates the Effects of High Fat, High Carb, and Pro/Anti-oxidative Treatments in Mice Public Deposited

http://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/bv73c1939

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  • Metabolic disorders affect much of the population, and it is widely believed that they have a genetic basis as well as being influenced by dietary factors. Recent studies have revealed an important role for the neil1 gene in the prevention of metabolic diseases. NEIL1 is a DNA Glycosylase that catalyzes the repair of DNA and mtDNA. We aimed to understand how a deletion in the neil1 gene in a mouse model would affect susceptibility to high fat and high carbohydrate diets. We also sought to establish a role for pro- and antioxidants in modulating disease risk. To accomplish this, Wild Type (WT) and neil1 Knockout (KO) mice were placed on either high fat or high carbohydrate diets. In a cohort of animals, the high fat diet was supplemented by the pro-oxidant KBrO3, or the antioxidant N acetyl cysteine. Under the oxidative stress of a high fat diet, the KO mice gained more weight than the WT mice. A similar difference was observed for the high carbohydrate treatment. The pro-oxidant KBrO3 seemed to mitigate the weight gain phenotype that was characteristic of a high fat diet, whereas the antioxidant NAC had no effect in both kinds of mice. At this point, the conclusion can be drawn that neil1-KO animals are more prone to the weight gain (and attendant effects of metabolic syndrome) induced by high fat and high carbohydrate diets.
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  • Abstract; Methods; Figure 1; Figure 2; Table 1; Figure 3; Table 2; Discussion; Literature Cited
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  • Original submission was Microsoft Powerpoint. Excel and Statplus used for producing original graphs. File was converted to .pdf using Microsoft PowerPoint for Mac 2011, Version 14.2.1.
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