Undergraduate Thesis Or Project
 

NEIL1 Deficiency Modulates the Effects of High Fat, High Carb, and Pro/Anti-oxidative Treatments in Mice

Public Deposited

Downloadable Content

Download file
https://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/bv73c1939

Descriptions

Attribute NameValues
Creator
Abstract
  • Metabolic disorders affect much of the population, and it is widely believed that they have a genetic basis as well as being influenced by dietary factors. Recent studies have revealed an important role for the neil1 gene in the prevention of metabolic diseases. NEIL1 is a DNA Glycosylase that catalyzes the repair of DNA and mtDNA. We aimed to understand how a deletion in the neil1 gene in a mouse model would affect susceptibility to high fat and high carbohydrate diets. We also sought to establish a role for pro- and antioxidants in modulating disease risk. To accomplish this, Wild Type (WT) and neil1 Knockout (KO) mice were placed on either high fat or high carbohydrate diets. In a cohort of animals, the high fat diet was supplemented by the pro-oxidant KBrO3, or the antioxidant N acetyl cysteine. Under the oxidative stress of a high fat diet, the KO mice gained more weight than the WT mice. A similar difference was observed for the high carbohydrate treatment. The pro-oxidant KBrO3 seemed to mitigate the weight gain phenotype that was characteristic of a high fat diet, whereas the antioxidant NAC had no effect in both kinds of mice. At this point, the conclusion can be drawn that neil1-KO animals are more prone to the weight gain (and attendant effects of metabolic syndrome) induced by high fat and high carbohydrate diets.
Resource Type
Date Available
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Non-Academic Affiliation
Rights Statement
Funding Statement (additional comments about funding)
  • Center for Research on Occupational and Environmental Toxicology
Publisher
Language
Replaces

Relationships

Parents:

This work has no parents.

In Collection:

Items

Thumbnail Title Date Uploaded Visibility Actions
file details Presentation Part 1.ppt 07/21/2017 Public
file details Presentation Part 2.ppt 07/21/2017 Public
file details Presentation Part 3.ppt 07/21/2017 Public
file details TripathiMaanas.pdf 07/21/2017 Public