Undergraduate Thesis Or Project
 

Grimwood BRR thesis.pdf

Public Deposited

Downloadable Content

Download PDF
Request Accessible Version
https://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/c534fq86r

Descriptions

Attribute NameValues
Creator
Abstract
  • Endogenous circadian clock regulation is essential to normal rhythmicity, particularly the timing of hormone release in the brain. In the context of mammalian reproduction, a surge of a specific hormone, gonadotrophin-releasing hormone (GnRH), initiates a surge of luteinizing hormone (LH) from the pituitary gland, which is required for ovulation in females. Ordinarily, GnRH is secreted in a pulsatile pattern distinct from the surging that promotes ovulation. These surges occur with an approximately 24-hour release pattern and require elevated levels of ovarian estradiol (E2), the most common form of estrogen in human mammals, originating from the granulosa cells of the developing follicle. GnRH neurons express the estrogen receptor isoform estrogen receptor β (ERβ). Currently, little is known about mechanisms underlying GnRH/LH surge timing and how E2 acts directly on GnRH neurons. To better understand how endogenous clocks interact with sex-steroid hormone signalling, we explored protein-protein interactions between ERβ and the circadian clock transcription factor BMAL1 in multiple representative cell lines, in both the absence and presence of E2. It was hypothesized that direct protein-protein interactions may exist among clock components and ERβ in immortalized GnRH neurons (GT1-7 cell lines), exhibiting altered interactions in the presence of E2. Co-Immunoprecipitation (Co-IP) and Western blot procedures provided unclear results as some data supported the hypothesis, while other data contradict it.
License
Resource Type
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Rights Statement
Publisher
Accessibility Feature

Relationships

Parents:

Items

Thumbnail Title Date Uploaded Visibility Actions
file details Grimwood_BRR_thesis.pdf 2017-07-28 Public Download